Effect of Bifidobacterium on the expression of β-defensin-2 in intestinal tissue of neonatal rats with necrotizing enterocolitis / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study the effect of Bifidobacterium on the expression of β-defensin-2 (BD-2) in intestinal tissue of neonatal rats with necrotizing enterocolitis (NEC).</p><p><b>METHODS</b>A total of 40 rats were randomly divided into four groups: normal control, Bifidobacterium control, NEC model, and Bifidobacterium treatment, with 10 rats in each group. A rat model of NEC was induced by hypoxia, cold stimulation, and artificial feeding. The rats in the Bifidobacterium control and Bifidobacterium treatment groups were given Bifidobacterium via the gastric tube after cold stimulation once a day for three consecutive days. The morphological changes of the terminal ileum were observed under a light microscope and the intestinal injury score was determined. Immunohistochemistry and qRT-PCR were used to measure the protein and mRNA expression of BD-2 in the ileal mucosal tissue.</p><p><b>RESULTS</b>The NEC model group had a significantly higher intestinal injury score than the normal control, Bifidobacterium control, and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium treatment group had a significantly higher intestinal injury score than the normal control and Bifidobacterium control groups (P<0.05). The mRNA and protein expression of BD-2 in the normal control group was significantly lower than in the Bifidobacterium control, NEC model, and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium control group had significantly higher mRNA and protein expression of BD-2 than the NEC model and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium treatment group had significantly higher mRNA and protein expression of BD-2 than the NEC model group (P<0.05).</p><p><b>CONCLUSIONS</b>Bifidobacterium can induce the expression of BD-2 in intestinal tissue of rats and reduce inflammatory response by increasing the expression of BD-2. This provides a protective effect on neonatal rats with NEC.</p>

Association between surfactant protein B gene polymorphisms and susceptibility to bronchopulmonary dysplasia in preterm infant / 中华实用儿科临床杂志

Objective To investigate the distribution of surfactant protein B (SP-B) gene single nucleotide polymorphisms and to study the association between the SP-B gene polymorphisms and bronchopulmonary dysplasia (BPD) in preterm infant.Methods Forty-two preterm infants with BPD and 68 preterm infants without BPD (control group) were selected.Polymerase chain reaction with restriction fragment length polymorphism was used to establish the genotype and allele frequencies of the SP-B-18A/C,SP-B 1580C/T,SP-B 8714G/C single nucleotide the polymorphisms,and then the association between the polymorphisms and BPD was analyzed.Results The frequencies of genotype CC,AC,AA of SP-B-18A/C in BPD group and control group were 35.7％,52.4％,11.9％ and 32.3％,47.7％,20.0％,respectively.The frequencies of genotype CC,GC,GG of SP-B 8714G/C in BPD group and the control group were 26.2％,54.8％,19.0％ and 27.7％,58.5％,13.8％,respectively.The frequencies of genotype CC,CT,TT of SP-B 1580C/T in BPD group and control group were 66.7 ％,26.2％,7.1％ and 40.0％,47.7 ％,12.3 ％,respectively.The frequencies of allele C and allele A of SP-B-18A/C in BPD group and control group were 61.9％,38.1％ and 56.2％,43.8％,respectively.The frequencies of allele G and allele C of SP-B 8714G/C in BPD group and control group were 53.6％,46.8％ and 56.9％,43.1％,respectively.The frequencies of allele C and allele T of SP-B 1580C/T in BPD group and control group were 79.8％,20.2％ and 63.8％,36.2％,respectively.SP-B-18A/C,SP-B 1580C/T,SP-B 8714G/C were found to be polymorphic.The frequencies of genotype CC of SP-B 1580C/T in BPD group were significantly higher than those in the controls (x2 =7.26,P ＜ 0.05).The frequencies of allele C of SP-B 1580C/T were also statistically different between BPD group and control group(x2 =6.17,P ＜0.05).The C allele increased the risk of BPD(OR =2.23,95％ CI:1.18-4.24).There was no significant difference between the 2 groups in the frequencies of genotype and allele of SP-B-18A/C and SP-B 8714G/C.Conclusions SP-B 1580C/T polymorphism has association with the etiology of BPD and may serve as the susceptibility gene for BPD,the C allele increase the risk of BPD.SP-B-18A/C and SP-B 8714G/C do not have association with the etiology of BPD.

Relationship between pulmonary surfactant-associated protein B polymorphisms and the susceptibility to neonatal respiratory distress syndrome / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study the relationship between pulmonary surfactant-associated protein B (SP-B) gene polymorphisms and their susceptibility to neonatal respiratory distress syndrome (RDS).</p><p><b>METHODS</b>Eighty-eight preterm infants with RDS (RDS group) and 103 infants without RDS (control group) were enrolled. The genomic DNA was isolated using DNA kits. Polymerase chain reaction with restriction fragment length polymorphism technique was used to detect the genotype and allele frequency of the SP-B -18A/C and SP-B 1580C/T single nucleotide polymorphisms. The association between the polymorphisms and RDS was analyzed.</p><p><b>RESULTS</b>SP-B -18A/C and SP-B 1580C/T were found to be polymorphic in both RDS and control groups. The frequencies of CC genotype (X2=12.26, P<0.01) and C allele (X2=11.97, P<0.01) of SP-B 1580C/T were significantly higher in the RDS group than in the control group. The C allele significantly increased the risk of RDS (OR=2.26, 95%CI: 1.42-3.60). The frequencies of genotype and allele of SP-B -18A/C showed no significant difference between the two groups.</p><p><b>CONCLUSIONS</b>SP-B 1580C/T polymorphism contributes to the etiology of RDS and may serve as the susceptibility gene for RDS. The C allele increases the risk of RDS. SP-B -18A/C shows no association with the etiology of RDS.</p>

Selection of the internal fixation for various types of intertrochanteric fracture in aged patients and prevention for complication / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the results and indication of differently internal fixation for intertrochanteric fracture and prevent for complication.</p><p><b>METHODS</b>One hundred and two patients of intertrochanteric fracture of femur from January 1997 to December 2007 were reviewed and analysed. There were 46 males and 56 females, with an average age of 76.5 years ranging from 60 to 93 years. There were 34 cases of stability fractures and 68 of instability fractures. The methods of internal fixation included dynamic hip screw in 82 cases, proximal interlocking nail in 5, multi-screw fixation in 9, dynamic codylar screw in 6.</p><p><b>RESULTS</b>These patients were followed up for 6 to 72 months (36 months in average). The time of fracture healing was from 8 to 12 weeks. According to evaluation criterion of effect, the results were excellent in 72 cases, good in 20, fair in 10. After operation, there were 3 cases of coax vara, 2 cases of lag screws cutting out, 1 case of breakage of screw, 2 cases of avascular necrosis of the femoral head, 1 case of postoperative infection, 1 case of delayed union.</p><p><b>CONCLUSION</b>Suitable implant selection according the type of fracture, health condition is an important factor to reduce the incidence of complication for unstable fracture in aged patients. DHS is a main treatment of intertrochanteric fracture. PFN is recommended for serious osteoporosis or unstable intertrochanteric fracture. Compression screws is a choice for the fixation of type I and type II fracture of bad health condition.</p>